Research Associate IDMPK

Key Responsibilities

In Vitro ADME Studies

• Read and understand various in vitro ADME protocols.
• Conduct in vitro stability experiments for new chemical entities (liver microsomes, plasma, and other relevant matrices, chemical stability).
• Perform in vitro ADME experiments such as:
• logP, logD, pKa measurements
• CYP induction and inhibition assays
• Permeability determinations (Caco-2, MDCK etc.) in relevant cell lines
• CYP phenotyping in purified enzyme systems
• Solubility determinations (kinetic, thermodynamic, simulated fluids)

Bioanalysis

• Read and understand the bioanalysis sections in the study protocols.
• Perform LC-MS/MS and HPLC-UV/PDA method development and validation (partial and complete).
• Develop fit-for-purpose LC-MS/MS methods for routine in vitro sample analysis.
• Extract data from the instrument, conduct bioanalytical QC, and share reports with DMPK stakeholders.
• Experience with method development in various matrices focused on in vitro samples is required; experience with plasma, blood, tissues, urine, feces, etc. is a plus.
• LC-MS/MS method development for biochemical and cell-based screening of NCEs is a plus but not mandatory.
• Perform general LC-MS/MS and HPLC maintenance and troubleshooting.

Data Analysis and Report Generation

• Understand the quality of bioanalytical data.
• Conduct data analysis using Phoenix WinNonlin (a plus but not mandatory).
• Calculate in vitro results/parameters using Excel spreadsheets.
• Generate reports in Excel, Word, and PowerPoint format and share with the line manager.
• Prepare reports in both GLP and non-GLP formats as per requirement.

Person Profile

• Education: M.Sc / M.Pharm
• Experience: 2–6 years in a drug discovery industry/CRO in the DMPK department